Which of the following cell types are not crucial for healing a clean, incisional wound?

D. Polymorphonuclear leukocyte.

E. Myofibroblast.

DISCUSSION: Experimental studies have shown that healing may progress normally in the absence of polymorphonuclear leukocytes in an uninfected wound. In contrast, depletion of monocytes and macrophages causes a severe alteration in wound healing with poor débridement, delayed fibroblast proliferation, and inadequate angiogenesis. Platelets carry a cadre of biologically active substances that are important for wound repair, including peptide growth factors like platelet-derived growth factor (PDGF) and TGF-b. Fibroblasts are the principal cell for matrix synthesis and deposition. Myofibroblasts are important for wound contraction in open defects but have little if any role in clean, incisional wounds.