Q:

A young man who has no past medical history presented with jaundice, low Hgb, reticulocytes 8% and other indices within normal limit but occasional spherocytes were seen on blood film. What is the single most appropriate investigation?

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A young man who has no past medical history presented with jaundice, low Hgb, reticulocytes 8% and other indices within normal limit but occasional spherocytes were seen on blood film. What is the single most appropriate investigation? 


  1. G6PD enzyme assay
  2. Direct coombs test
  3. Repeat blood film
  4. Indirect coombs test
  5. BMA

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The key is B. Direct Coombs test. [The direct Coombs test, is used to determine whether the cause of hemolytic anemia, is due to antibodies attached to RBCs which are seen in autoimmune-related hemolytic anemia].

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